Stem cell technology serves as a core pillar in regenerative medicine and precision medicine, and the safety and standardization of its research and application directly depend on the construction level of specialized laboratories. The construction of stem cell laboratories differs from that of ordinary biological laboratories, requiring consideration of four core dimensions: clean environment control, biosafety protection, equipment precision assurance, and a quality management system. It must strictly adhere to relevant national and industry standards, achieving standardization throughout the entire process from site planning to operation and maintenance management. This article, based on relevant industry standards, elaborates on the core points and implementation paths for stem cell laboratory construction, providing a practical construction reference for research institutions, medical institutions, and enterprises.