PCR Laboratory in Bonn, Germany
PCR Laboratory Case
Location: Bonn, Germany
Project Name: PCR Laboratory
Cleanliness Class: Class 100,000 (ISO 8) | Wall Structure: Magnesium oxide (MGO) color steel panels
HVAC & Cleanroom Equipment: Central cleanroom HVAC system, purified water system, pass-through boxes
Instrumentation: Venturi valves, differential pressure gauges
Piping Systems: Stainless steel purified water system, ductwork, drainage system
Flooring: PVC flooring
Electrical Systems: Power distribution cabinets, cleanroom lighting, switches and sockets, UV lamps, ozone system
In principle, a PCR laboratory consists of four distinct work areas—reagent preparation, sample preparation, amplification, and amplification product analysis—served by a dedicated corridor. Buffer rooms should be installed for each work area, and interlocking mechanisms should be fitted between the work areas and their respective buffer rooms. Functionally distinct areas must be physically separated and clearly marked; direct access between them is prohibited, and pass-through boxes must be installed if they are adjacent. The first two areas constitute the pre-amplification zone, while the latter two constitute the post-amplification zone; these zones must be strictly segregated. Materials, reagents, records, stationery, and cleaning supplies must flow only from the pre-amplification zone to the post-amplification zone (i.e., reagent preparation → sample preparation → amplification → product analysis) with no reverse flow.
Unidirectional flow—covering experimental processes, materials, personnel, and air—must be maintained between areas to create a protective barrier, prevent cross-interference, and avoid contamination caused by aerosol dispersion. The laboratory enclosure structure must be robust and airtight; all internal corners (both concave and convex) must feature coved (radiused) transitions. Interior wall surfaces must be smooth, dust-resistant, corrosion-resistant, and easy to clean and disinfect. Flooring should consist of PVC sheet flooring or self-leveling epoxy resin, meeting requirements for seamlessness, leak-proof integrity, smooth finish, and corrosion resistance.